Airway epithelial cells cultured on a Transwell membrane.  NaK-ATPase (green) is on the basolateral membrane.  Nuclei are labeled with Propidium Iodide (red).  Cilia (gray) above apical membrane (light gray) are imaged using DIC.    Tissue prepared and imaged on a LeicaSP2 confocal microscope by Dr. A. Livraghi. NaK-ATPase (green) is on the basolateral membrane. (Click on image above for full size view)

DIC only view from above.  Gamma corrected.